The State of the Art in Transcriptome Analysis RNA sequencing (RNA-seq) data offers unprecedented information about the transcriptome but harnessing this information with bioinformatics tools is typically a bottleneck RNA-seq Data Analysis A Practical Approach enables researchers to examine differential expression at gene exon & transcript levels & to discover novel genes transcripts & whole transcriptomes Balanced Coverage of Theory & Practice Each chapter starts with theoretical background followed by descriptions of relevant analysis tools & practical examples Accessible to both bioinformaticians & nonprogramming wet lab scientists the examples illustrate the use of comm&-line tools R & other open source tools such as the graphical Chipster software The Tools & Methods to Get Started in Your Lab Taking readers through the whole data analysis workflow this self-contained guide provides a detailed overview of the main RNA-seq data analysis methods & explains how to use them in practice It is suitable for researchers from a wide variety of backgrounds including biology medicine genetics & computer science The book can also be used in a graduate or advanced undergraduate course ...

Founded in 1824 the RNLI is a unique charity Funded by public donations it provides a 247 search & rescue service around the coastlines of the UK & Republic of Ireland It designs & builds its own lifeboats & the vast majority of its lifeboat crew members are volunteers
- ordinary people from all walks of life who give up their time to help others As well as lifeboats the RNLI provides a beach lifeguard service The charity also works to improve water safety through education & awareness campaigns as well as sharing its expertise with life-saving organisations overseas There are over 350 lifeboat in the RNLI fleet based at our stations around the UK & Ireland Our RNLI gift pack combines three of the most iconic- The Severn Class Lifeboat (1173)
- the largest lifeboat in the fleet Length 98mm
- The Shannon Class Lifeboat (1136)
- the most agile & manoeuvrable all-weather lifeboat Length 100mm
- The Land Rover Defender & D Class Lifeboat (183)
- The D class inshore lifeboat has been the workhorse of the RNLI for over 50 years Length 135mm





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Please note this is a region B Blu-ray & will require a region B or region free Blu-ray player in order to play Documentary following two sets of brothers; Robert & Joey Dunlop & Robert&s sons William & Michael Between the four of them they are some of the most successful road racers in the world Narrated by Liam Neeson & featuring never-before-seen action ROAD tells the story of two generations of success ...

A volume of short stories by the bestselling author of THE BROKEN EMPIRE series Mark Lawrence This is a collection of fourteen stories of murder mayhem pathos & philosophy all set in the world of the Broken Empire Within these pages you will find tales of men such as Red Kent Sir Makin Rike Burlow & the Nuban telling of their origins & the events that forged them There is Jorg himself striding the page as a child of six as a teenage wanderer & as a young king & then there is a tale about Prince Jalan Kendeth
- liar cheat womaniser & coward To the new reader welcome to a lawless world where wit & sword are the most useful weapons & danger lurks as much in candle-lit palaces as in dark alleys & dense woodland To those who have already journeyed with Jorg we hope you will enjoy renewing old acquaintances with your favourite characters
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Please note this is a region 2 DVD & will require a region 2 (Europe) or region Free DVD Player in order to play Documentary following two sets of brothers; Robert & Joey Dunlop & Robert&s sons William & Michael Between the four of them they are some of the most successful road racers in the world Narrated by Liam Neeson & featuring never-before-seen action ROAD tells the story of two generations of success ...

Please note this is a region 2 DVD & will require a region 2 (Europe) or region Free DVD Player in order to play Roadhouse From the producer of Die Hard and Lethal Weapon comes a sexy action-packed adventure that pushes the envelope for high-octane thrills Patrick Swayze stars as one of the screen’s most unforgettable heroes charged with magnetic charm & lightning-fast martial-arts moves Dalton (Swayze) is a legendary bouncer who comes to Jasper Missouri for a special purpose to restore order at the dangerous Double Deuce bar But as one by one he rids the bar of thugs hoodlums & hit men he incurs the wrath of their employer--the notorious racketeer Brad Wesley (Ben Gazzara) The town lives in fear of Wesley but Dalton is ready for action! He confronts Wesley’s goons in one spectacular fight after another but when they strike back with deadly force Dalton knows he must face the crime boss himself in a blistering battle to the finish Co-starring Kelly Lynch (Cocktail) & Sam Elliott (Gettysburg) & featuring hard-driving music by Alabama the Jeff Healey Band Otis Redding & more Road House is where the action is Roadhouse 2 Shane Tanner (Johnathon Schaech) is an undercover DEA agent with all the right moves He’s put away his share of dealers but hasn’t made a big-time bust When a deadly drug runner begins terrorising his uncle Nate’s bar in Louisiana Shane leaves New York & heads south determined to settle the score From drunken fistfights to all-out brawls the Black Pelican is known for its rough & rowdy atmosphere It’s also considered prime real estate for drug trafficking--and one local dealer Wild Bill (Jake Busey) is determined to make the road house his personal office by any means necessary But when Shane takes over the Pelican for his battered uncle he’s about to find out that this local drug ring is bigger & deadlier than he ever imagined With help from a gorgeous blonde (Ellen Hollman) & backup from the DEA Shane is out to make the biggest bust of his career & exact a lethal shot of revenge along the way ...

Please note this is a region 2 DVD & will require a region 2 (Europe) or region Free DVD Player in order to play Patrick Swayze stars as Dalton in this modern-day western as a tough guy with poetic instincts & a Mercedes Fresh from earning his degree in Eastern Philosophy from New York University mettlesome loner Dalton finds work as a bouncer at a violent road house Within weeks Dalton has rid the bar of the trouble-makers ruining its business & has even struck up a romance with a sexy doctor When Dalton bounces the wrong man who turns out to be one of local crime boss Brad Wesley&s henchmen Wesley first tries to buy Dalton off but fails When Wesley learns of Dalton&s new lover who used to be Wesley&s significant other Wesley vows bloody vengeance But Dalton with his unique mixture of brute force & calmness isn&t about to let his college money go to waste Actors Patrick Swayze Ben Gazzara Kelly Lynch & Sam Elliott Director Rowdy Herrington Certificate 18 years & over Year 1989 Screen Widescreen 2351 Anamorphic Languages English ; Spanish ; Italian ; French ; German
- Dolby (20) Stereo Subtitles French ; Italian ; Spanish ; Dutch ; Swedish ; Norwegian ; Danish ; English For Hearing Impaired ; German For The Hearing Impaired Duration 1 hour & 49 minutes (approx) Region Region 2
- Will only play on European Region 2 or multi-region DVD players

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THE SUNDAY TIMES NUMBER-ONE BESTSELLER Michael Dunlop is quite simply the greatest road racer on the planet Brother of William also an accomplished rider son of the late Robert & nephew of the late great Joey Dunlop Michael can fairly claim that racing is in his blood Now for the first time he talks in depth about his family story how he got involved in the family business & how he manages to keep getting back on his bike despite all he knows of the deadly risks he encounters every time he crosses the start line The death of his uncle during a competition in Estonia in 2000 was followed just eight years later by the death of his father at the North West 200 But despite these tragic losses Michael was undeterred & two days after his father&s death he returned to the North West & won The next year Michael won his first TT joining both his father & uncle in the record books Now with thirteen TT wins to his name Michael is a phenomenal competitor & in this sensational autobiography he reveals the highs & lows of racing what it was like growing up part of a motorcycle dynasty & how that made him the incredible racing driver he is today ...

Acclaimed novelist Eisner-winning graphic novelist Joe Hill collaborated with his father Stephen King in Throttle for the first time on a tale that paid tribute to Richard Matheson's classic tale Duel Now IDW is proud to present comic-book tellings of both stories in Road Rage adapted by Chris Ryall with art by Nelson Daniel & Rafa Garres ...

On the isolated road of the American highway terror rides on 18 wheels! Tales of diesel-fueled fear from three masters of horror fiction With Throttle acclaimed novelist Eisner-winning graphic novelist Joe Hill collaborated with his father Stephen King for the first time on a tale that paid tribute to Richard Matheson&s classic short story Duel Now IDW is proud to present comic book versions of both stories in Road Rage adapted by Chris Ryall with art by Nelson Daniel & Rafa Garres About the Author Joe Hill is the 1 New York Times Bestselling Author of The Fireman NOS4A2 Heart-Shaped Box & Horns (which was made into a feature film starring Daniel Radcliffe) His book of short stories 20th Century Ghosts won the Bram Stoker Award & British Fantasy Award for Best Collection He earned the Eisner Award for Best Writer for his long-running comic book series Locke & Key featuring the eye-popping art of Gabriel Rodriguez Stephen King was born in Portland Maine in 1947 the second son of Donald and Nellie Ruth Pillsbury King He made his first professional short story sale in 1967 to Startling Mystery Stories In the fall of 1973 he began teaching high school English classes at Hampden Academy the public high school in Hampden Maine Writing in the evenings & on the weekends he continued to produce short stories & to work on novels In the spring of 1973 Doubleday & Co accepted the novel Carriefor publication providing him the means to leave teaching & write full-time He has since published over 50 books & has become one of the world&s most successful writers Stephen lives in Maine & Florida with his wife novelist Tabitha King They are regular contributors to a number of charities including many libraries & have been honored locally for their philanthropic activities Richard Matheson was the New York Times bestselling author of I Am Legend Hell House Other Kingdoms Somewhere in Time The Incredible Shrinking Man A Stir of Echoes The Beardless Warriors The Path Seven Steps to Midnight Now You See It… & What Dreams May Come among others He was named a Grand Master of Horror by the World Horror Convention & received the Bram Stoker Award for Lifetime Achievement He also won the Edgar the Spur & the Writer&s Guild awards In 2010 he was inducted into the Science Fiction Hall of Fame In addition to his novels Matheson wrote screenplays including several Twilight Zone episodes such as “ Nightmare at 20000 Feet” based on his short story He was born in New Jersey & raised in Brooklyn & fought in the infantry in World War II He earned his bachelor’s degree in journalism from the University of Missouri He passed away in 2013 Chris Ryall is the Chief Creative Officer at IDW Publishing & the co-creator of Zombies vs Robots with Ashley Wood Ryall has also co-created & written Groom Lake The Colonized The Hollows & Onyx & has also written adaptations of stories by Stephen King Clive Barker & Richard Matheson He is also the co-author of a prose book about comics Comic Books 101 & has written comics based on the Transformers the band Kiss Mars Attacks Weekly World News & currently Hasbro’s Rom Ryall & Zv R co-creator Ashley Wood were nominated for the 2006 Eisner Award for “ Best Short Story” ...