Microbiology of food & animal feed. Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens. Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) & the determination of O157, O111, O26, O103 & O145 serogroups Food products, Food technology, Animal feed, Microbiological analysis, Microorganisms, Laboratories, Laboratory testing, Laboratory equipment, Test equipment, Laboratory techniques, Occupational safety, Hygiene, Personal hygiene, Sterilization (hygiene), Decontamination, Culture techniques, Count methods (microbiology), Test specimens, Statistical methods of analysis, Mathematical calculations, Confidence limits